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High-throughput protein expression

Midwest Center for Structural Genomics

Two advances in high-throughput protein expression are discussed: a vector and supplemented minimal media for production of selenomethionyl protein derivatives. The vector produces fusion proteins in which MBP, the his(6)-tag, and the target protein are separated by highly specific protease cleavage sites. E. coli express improved yields of selenomethionyl derivatives of soluble proteins at low temperatures in the newly formulated minimal media.

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GNF96 - A new minimal coarse screen for crystallography

Joint Center for Structural Genomics

An analysis of over 340,000 individual crystallization trials has led to the creation of a new minimal coarse screen (GNF96), which is highly effective in identifying targets which crystallize easily and providing leads for optimization.

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AUTOPROC

Northeast Structural Genomics Consortium

AutoProc is a suite of programs for the automatic generation of scripts for multidimensional protein NMR data processing and format conversion.

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AutoStructure

Northeast Structural Genomics Consortium

AutoStructure is a protein structure determination tool that uses uninterpreted NOESY cross peaks together with structure calculation programs like XPLOR or DYANA to generate a 3D structure.

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AutoAssign

Northeast Structural Genomics Consortium

AutoAssign is a software package for automated analysis of backbone resonance assignments from triple-resonance NMR data of proteins.

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