Center for Eukaryotic Structural Genomics:


A global optimization method for the molecular replacement problem in X-ray crystallography

Center for Eukaryotic Structural Genomics

We introduce a new global optimization strategy that combines a coarse-grid search, using a surrogate function, with extensive multi-start local optimization. A new MR code, called SOMoRe, based on this strategy is developed and tested on four realistic problems, including two difficult problems that traditional MR codes failed to solve directly.

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A phyloproteomic characterization of in vitro autophosphorylation in calcium-dependent protein kinases

Center for Eukaryotic Structural Genomics

Calcium-dependent protein kinases (CDPKs) are a novel class of signaling molecules that have been broadly implicated in relaying specific calcium-mediated responses to biotic and abiotic stress as well as developmental cues in both plants and protists.

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A wheat germ cell-free expression system for SeMet labelling

Center for Eukaryotic Structural Genomics

We have shown that the wheat germ cell-free protein production platform used by CESG can be used for efficient production of [Se-Met]-labeled proteins with incorporation efficiency very close to 100%.

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ACMI - Automatic interpretation of electron density maps

Center for Eukaryotic Structural Genomics

We describe ACMI (Automatic Crystallographic Map Interpreter), which uses a probabilistic model known as a Markov field to represent the protein. Residues of the protein are modeled as nodes in a graph, while edges model pairwise structural interactions.

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Abscisic acid inhibits type 2C protein phosophatases via the PYR/PYL family of START proteins

Center for Eukaryotic Structural Genomics

We show that ABA binds to PYR1, which in turn binds to and inhibits PP2Cs. We conclude that PYR/PYLs are ABA receptors functioning at the apex of a negative regulatory pathway that controls ABA signaling by inhibiting PP2Cs.

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