Center for Membrane Proteins in Infectious Diseases:


Biological and biochemical characterization of HIV-1 Gag/dgp41 virus-like particles expressed in Nicotiana benthamiana

Center for Membrane Proteins in Infectious Diseases

Plant-optimized HIV-1 genes were constructed and expressed in Nicotiana benthamiana. Our results demonstrate plant cells could support the formation of HIV-1 virus-like particles, but also the accumulation of these particles that incorporated dgp41.

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Crystallizing membrane proteins for structural and functional studies using lipidic mesophases. The power of the host lipid screen.

Center for Membrane Proteins in Infectious Diseases

Screening for crystallisability based on the identity of the lipid creating the hosting mesophase would be worthwhile. Host lipid screening is likely to prove a generally useful strategy for mesophase-based crystallisation of membrane proteins.

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Harvesting and cryo-cooling crystals of membrane proteins grown in lipidic mesophases for structure determination by macromolecular crystallography

Center for Membrane Proteins in Infectious Diseases

The use of lipidic mesophases, both cubic and sponge phases, for growing integral membrane crystal proteins has grown in popularity. Protocols for harvesting and cryo-cooling crystals grown in these two different phases are described in detail in this JoVE article (Li, et al., 2012).

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Host Lipid and Temperature as Important Screening Variables for Crystallizing Integral Membrane Proteins in Lipidic Mesophases. Trials with Diacylglycerol Kinase.

Center for Membrane Proteins in Infectious Diseases

A systematic study of the crystallization of an α-helical, integral membrane enzyme, diacylglycerol kinase, DgkA, using the lipidic cubic mesophase or in meso method has resulted in recommended screening strategies for crystallizing membrane proteins.

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In vivo protein crystallization

Center for Membrane Proteins in Infectious Diseases

We prepared nano-sized in vivo–grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.

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