Center for Membrane Proteins of Infectious Diseases:


Femtosecond X-ray protein nanocrystallography

Center for Membrane Proteins of Infectious Diseases

Here we present a method for structure determination where single-crystal X-ray diffraction 'snapshots' are collected from a fully hydrated stream of nanocrystals using femtosecond pulses from a hard-X-ray free-electron laser, the Linac Coherent Light Source. We prove this concept with nanocrystals of photosystem I, one of the largest membrane protein complexes.

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Lipidic phase membrane protein serial femtosecond crystallography

Center for Membrane Proteins of Infectious Diseases

We recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

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Toward structure determination using membrane-protein nanocrystals and microcrystals

Center for Membrane Proteins of Infectious Diseases

This work has been focused on showing the first proof of concept for using membrane protein nanocrystals and microcrystals for high-resolution structure determination.

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