Center for the X-ray Structure Determination of Human Transporters:


A gene optimization strategy that enhances production of fully functional P-glycoprotein in Pichia pastoris

Center for the X-ray Structure Determination of Human Transporters

Structural and biochemical studies of mammalian membrane proteins remain hampered by inefficient production of pure protein. We explored codon optimization based on highly expressed Pichia pastoris genes to enhance co-translational folding and production of P-glycoprotein (Pgp), an ATP-dependent drug efflux pump involved in multidrug resistance of cancers.

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