Chaperone-Enabled Studies of Epigenetic Regulation Enzymes:


A portable RNA sequence whose recognition by a synthetic antibody facilitates structural determination

Chaperone-Enabled Studies of Epigenetic Regulation Enzymes

Seeking to exploit antibody fragments as RNA crystallization chaperones, we have used an arginine-enriched synthetic Fab library displayed on phage to obtain Fabs against the class I ligase ribozyme. The resulting portable epitope provides a new RNA crystallization chaperone system that easily can be screened in parallel to the U1A RNA-binding protein, with the advantages of a smaller loop and Fabs' high molecular weight, large surface area and phasing power.

Read more





Generating conformation-specific synthetic antibodies to trap proteins in selected functional states

Chaperone-Enabled Studies of Epigenetic Regulation Enzymes

A detailed set of phage display sorting strategies and validation methodologies are presented that are capable of producing high performance synthetic antibodies (sABs) with customized properties.

Read more





Substance P derivatives as versatile tools for specific delivery of various types of biomolecular cargo

Chaperone-Enabled Studies of Epigenetic Regulation Enzymes

We synthesized variants of substance P (SP), an eleven amino acid neuropeptide ligand of the neurokinin type 1 receptor (NK1R), for the delivery of various types of cargo into cells.

Read more