Integrated Center for Structure and Function Innovation:


Annotating proteins with generalized functional linkages

Integrated Center for Structure and Function Innovation

Here, we propose a computational method of assignment of function, termed Generalized Functional Linkages (GFL), that combines nonhomology-based methods with other types of data.

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Automated, high-throughput platform for protein solubility screening using a split-GFP system

Integrated Center for Structure and Function Innovation

Here, we describe our current and on-going efforts to move the process of protein solubility screening from the bench (manual sample manipulation) to an automated, high-throughput, liquid-handling platform. We discuss optimization and validation of bacterial culture growth, lysis protocols, protein extraction, and assays of soluble and insoluble protein in multiple 96-well plate format. The optimized liquid-handling protocol can be used for rapid determination of the optimal, compact domains from single ORFS, collections of ORFS, or cDNA libraries.

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Chaperones to assist in crystallizing difficult protein and RNA targets

Integrated Center for Structure and Function Innovation

We have developed and implemented a high throughput process to produce crystallization chaperones to assist in crystallizing difficult protein and RNA targets.

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High throughput ligand analysis and selection for crystallization

Integrated Center for Structure and Function Innovation

To improve the success rate in crystallization, we have developed a ligand-protein interaction screen in collaboration with a group from UC-Riverside.

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Integrated Center for Structure and Function Innovation (ISFI)

Integrated Center for Structure and Function Innovation

Integrated Center for Structure and Function Innovation (ISFI) - http://techcenter.mbi.ucla.edu/

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