Membrane Protein Structures by Solution NMR:


A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems

Membrane Protein Structures by Solution NMR

We present a systematic tag variation strategy for the rapid improvement of cell-free expression efficiencies of membrane proteins based on the optimization of translation initiation.

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Application of iterative soft thresholding for fast reconstruction of NMR data non-uniformly sampled with multidimensional Poisson Gap scheduling

Membrane Protein Structures by Solution NMR

Here we present a fast implementation of the Iterative Soft Thresholding approach (istHMS) that can reconstruct high-resolution non-uniformly sampled NMR data up to four dimensions within a few hours and make routine reconstruction of high-resolution NUS 3D and 4D spectra convenient.

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Bicelles at low concentrations

Membrane Protein Structures by Solution NMR

Different detergent/lipid mixtures have been explored, leading to the identification of two different families of bicelles for which it is possible to lower the total amphiphile (detergent + lipid) concentration to <1% (w/v) while retaining isotropic assemblies.

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Cell-free expressed bacteriorhodopsin in different soluble membrane mimetics: biophysical properties and NMR accessibility

Membrane Protein Structures by Solution NMR

Selecting a suitable membrane-mimicking environment is of fundamental importance for the investigation of membrane proteins. Here, we present a comparative study of the heptahelical membrane protein bacteriorhodopsin in detergent micelles, amphipols, and nanodiscs.

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Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers

Membrane Protein Structures by Solution NMR

This review presents the current state-of-the-art of this technology and summarizes parameters that have been indicated as important for the co-translational association of cell-free synthesized membrane proteins with supplied membranes.

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