Mitochondrial Protein Partnership:


Differential scanning flourimetry for identification of protein ligands and stabilizing conditions at MPP

Mitochondrial Protein Partnership

Differential Scanning Fluorimetry (DSF) was used to demonstrate binding by the mitochondrial protein CPT1a of its physiological ligand carnitine, complex formation by trypsin and trypsin inhibitor, and metal ion binding by mitochondrial protein C20orf7.

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The use of PIPE cloning at MPP

Mitochondrial Protein Partnership

Polymerase Incomplete Primer Extension (PIPE) cloning is a ligase free cloning technology developed at the Joint Center for Structural Genomics (1,2). We use PIPE cloning extensively for the initial creation of expression clones. When the clones are made, PIPE technology is also used to modify them by deleting or exchanging tag or linker sequences, creating truncation variants, and making point mutations.

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