Northeast Structural Genomics Consortium:


A bidirectional system for the dynamic small molecule control of intracellular fusion proteins

Northeast Structural Genomics Consortium

Recently, we developed a method to degrade proteins fused to a mutant dehalogenase (HaloTag2) using small molecule hydrophobic tags (HyTs). Here, we introduce a complementary method to stabilize the same HaloTag2 fusion proteins, resulting in a unified system allowing bidirectional control of cellular protein levels in a temporal and dose-dependent manner.

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A novel strategy for NMR resonance assignment and protein structure determination

Northeast Structural Genomics Consortium

In order to facilitate NMR structure determination, we developed a semi-automated strategy that utilizes non-uniform sampling (NUS) and multidimensional decomposition (MDD) for optimal data collection and processing of selected, high resolution multidimensional NMR experiments, combined it with an ABACUS protocol for sequential and side chain resonance assignments, and streamlined this procedure to execute structure and refinement calculations in CYANA and CNS, respectively. Two graphical user interfaces (GUIs) were developed to facilitate efficient analysis and compilation of the data and to guide automated structure determination.

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AUTOPROC

Northeast Structural Genomics Consortium

AutoProc is a suite of programs for the automatic generation of scripts for multidimensional protein NMR data processing and format conversion.

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AutoAssign

Northeast Structural Genomics Consortium

AutoAssign is a software package for automated analysis of backbone resonance assignments from triple-resonance NMR data of proteins.

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AutoStructure

Northeast Structural Genomics Consortium

AutoStructure is a protein structure determination tool that uses uninterpreted NOESY cross peaks together with structure calculation programs like XPLOR or DYANA to generate a 3D structure.

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