Partnership for Stem Cell Biology:


Robust conditions for His-tag removal from transcription factors or transcription factor DNA binding domains

Partnership for Stem Cell Biology

Researchers at the Partnership for Stem Cell Biology have developed a reliable method for removing His-tags from transcription factors or transcription factor DNA binding domains. In the past, it has been problematic to remove His-tags from these full-length proteins or protein constructs, since they tended to be unstable after purification on Ni-beads. In conjunction with JCSG, they have used this method to assist in the structure determination of a novel winged-helix like domain from human nuclear factor related to kappa-B-binding protein (NFRKB).

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