Transmembrane Protein Center:


A Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesicles

Transmembrane Protein Center

The utility of this approach as a delivery method for production and incorporation of monotopic (peripheral) membrane proteins is discussed.

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A combined approach to improving large-scale production of tobacco etch virus protease

Transmembrane Protein Center

Tobacco etch virus NIa proteinase (TEV protease) is an important tool for the removal of fusion tags from recombinant proteins.

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Activity and topology determination of in vitro expressed membrane proteins

Transmembrane Protein Center

We report reconstitution of a three-component membrane protein complex using wheat germ cell-free technology (Goren and Fox, 2008).

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Amino acid determinants of substrate selectivity in the Trypanasoma brucei sphingolipid synthase family

Transmembrane Protein Center

The substrate selectivity of four Trypanosoma brucei sphingolipid synthases is examined.

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Cell-free production of integral membrane aspartic acid proteases reveals zinc-dependent methyltransferase activity of the Pseudomonas aeruginosa prepilin peptidase PilD

Transmembrane Protein Center

Integral membrane aspartic acid proteases are receiving growing recognition for their fundamental roles in cellular physiology of eukaryotes and prokaryotes, and may be medically important pharmaceutical targets.

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