Transmembrane Protein Center:


Cell-free synthesis and functional characterization of sphingolipid synthases from parasitic trypanosomatid protozoa

Transmembrane Protein Center

The Trypanosoma brucei genome has four highly similar genes encoding sphingolipid synthases (TbSLS1-4).

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Cell-free translation of integral membrane proteins into unilamelar liposomes

Transmembrane Protein Center

Wheat germ cell-free translation is shown to be an effective method to produce integral membrane proteins in the presence of unilamelar liposomes.

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Co-translation of integral membrane proteins (IMP) with membrane scaffold proteins (MSP), also known as nanodiscs

Transmembrane Protein Center

Membrane protein solubilization is achieved using wheat-germ cell-free translation by co-expression of the target membrane protein with a membrane scaffold protein derived from apolipoprotein in the presence of excess lipids. Two transcripts are added in varying ratios to optimize the co-translational formation of the soluble MSP/membrane protein complex. This complex can be purified using an affinity tag on either the IMP or the MSP. Soluble IMP/MSP complexes can be used for functional studies, characterization of membrane protein complexes, and the development of crystallography reagents such as synthetic antibody scaffolds.

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Customized expression vector platform

Transmembrane Protein Center

A series of expression vectors used for protein production and different research activities are available.

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Detergent solubilization screening for membrane proteins expressed in E. coli

Transmembrane Protein Center

We have developed new purification methods for membrane proteins expressed in Escherichia coli (E. coli), that is based on a high-throughput detergent solubilization screen and IMAC protein purification.

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