Transmembrane Protein Center:


Development of detergent arrays for membrane protein compatibility screening

Transmembrane Protein Center

We have developed a series of detergent arrays for high-throughput screening of cell-free protein expression of membrane proteins.

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Differential scanning flourimetry for identification of protein ligands and stabilizing conditions at TMPC

Transmembrane Protein Center

Differential Scanning Fluorimetry (DSF) was used to demonstrate binding by the mitochondrial protein CPT1a of its physiological ligand carnitine, complex formation by trypsin and trypsin inhibitor, and metal ion binding by mitochondrial protein C20orf7.

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Expression and purification of bacteriorhodopsin using Protemist XE with detergents

Transmembrane Protein Center

The use of the Protemist XE, an automated discontinuous-batch protein synthesis robot, in cell-free translation is reported.

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Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes

Transmembrane Protein Center

Voltage-gated ion channels are a class of membrane proteins that temporally orchestrate the ion flux critical for chemical and electrical signaling in excitable cells. Here, we describe a new integrated approach combining cell-free translation of membrane proteins and in vivo expression using Xenopus laevis oocytes.

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In vivo inactivation of mycobacterial integral membrane stearoyl-CoA desaturase DesA3 by a C-terminal specific degradation process

Transmembrane Protein Center

DesA3 (Rv3229c) from Mycobacterium tuberculosis is a membrane-bound stearoyl coenzyme A delta-9-desaturase that reacts with the oxidoreductase Rv3230c to produce oleic acid. This work provides evidence for a mechanism used by mycobacteria to regulate this essential enzyme activity. DesA3 expressed as a fusion with either a C-terminal His(6) or c-myc tag had consistently higher activity and stability than native DesA3 having the native C-terminal sequence of LAA, which apparently serves as a binding determinant for a mycobacterial protease/degradation system directed at DesA3.

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