Transmembrane Protein Center:


Maltose–neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins

Transmembrane Protein Center

The Maltose-Neopentyl Glycol (MNG) amphiphiles outperform convententional detergents for stabilizing and extracting integral membrane proteins.

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One-plasmid tunable co-expression for mycobacterial protein-protein interaction studies

Transmembrane Protein Center

A single plasmid that allows controlled coexpression has been developed for use in mycobacteria. The tetracycline inducible promoter, PtetO, was used to provide tetracycline-dependent induction of one gene, while the Psmyc, Pimyc, or Phsp promoters were used to provide three different levels of constitutive expression of a second gene.

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Optimization of membrane protein production in E. coli and robotic purification

Transmembrane Protein Center

Several membrane proteins have been successfully expressed in E. coli and purified using Maxwell robotic protein purification systems.

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Robotic large-scale application of wheat cell-free translation to structural studies including membrane proteins

Transmembrane Protein Center

The use of the Protemist XE, an automated discontinuous-batch protein synthesis robot, in cell-free translation is reported.

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Structural genomics methods applied to production of the monotopic membrane protein human cytochrome b5 and in situ delivery to liposomes

Transmembrane Protein Center

Fusion protein vectors developed for high-throughput protein expression as part of the Protein Structure Initiative have been investigated for use in the expression and stabilization of human cytochrome b5, a monotopic membrane protein that must be attached to the cellular membrane for function.

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