Lipidic phase membrane protein serial femtosecond crystallography

Technology

Crystallography

Summary

We recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

Description

X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. We used crystals grown in the lipidic sponge phase, which is derived from the lipidic cubic phase by swelling. Micrometer-sized lipidic sponge phase crystals of a new crystal form of the Blastochloris viridis photosynthetic reaction center were delivered into an X-FEL beam using a sponge phase micro-jet. We recorded interpretable diffraction data to solve a structure of this new crystal form to 0.82 nanometer resolution using molecular replacement.

The April 2012 PSI SBKB Technical Highlight describing this technology can be found at http://sbkb.org/update/research/smaller-may-be-better.

Publication

Johansson LC, Arnlund D, White TA, Katona G, Deponte DP, Weierstall U, Doak RB, Shoeman RL, Lomb L, Malmerberg E, Davidsson J, Nass K, Liang M, Andreasson J, Aquila A, Bajt S, Barthelmess M, Barty A, Bogan MJ, Bostedt C, Bozek JD, Caleman C, Coffee R, Coppola N, Ekeberg T, Epp SW, Erk B, Fleckenstein H, Foucar L, Graafsma H, Gumprecht L, Hajdu J, Hampton CY, Hartmann R, Hartmann A, Hauser G, Hirsemann H, Holl P, Hunter MS, Kassemeyer S, Kimmel N, Kirian RA, Maia FR, Marchesini S, Martin AV, Reich C, Rolles D, Rudek B, Rudenko A, Schlichting I, Schulz J, Seibert MM, Sierra RG, Soltau H, Starodub D, Stellato F, Stern S, StrĂ¼der L, Timneanu N, Ullrich J, Wahlgren WY, Wang X, Weidenspointner G, Wunderer C, Fromme P, Chapman HN, Spence JC, Neutze R. "Lipidic phase membrane protein serial femtosecond crystallography." Nat Methods. 9:263-5 (2012). PubMed ID: 22286383 | Search SBKB Publications portal | PMC Link

Contact

Petra Fromme; Email: pfromme@asu.edu

Center for Membrane Proteins of Infectious Diseases

Link




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Last edited:Tue 24 Jun 2014 - 2 years, 10 months ago