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Automating the Structure Determination Pipeline: Advances by Two Membrane Protein Centers


3PJZ In the burgeoning field of membrane protein structure determination, two of the PSI:Biology Centers for Membrane Protein Structure Determination have developed automation and robotic technology to establish efficient and productive structure determination pipelines. Since these two centers ultimately use different methods to solve structures, the steps along the pathways are divergent. To reach the common goal of membrane protein structure determination, the Transcontinental EM Initiative for Membrane Protein Structure (TEMIMPS) employs electron microscopy and the New York Consortium on Membrane Protein Structure (NYCOMPS) primarily uses x-ray crystallography and NMR spectroscopy. These centers have optimized different aspects of their high throughput structure determination pipelines, automating many of the steps along the pathway from target selection to structure determination.

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TEMIMPS

TEMIMPS
NYCOMPS

NYCOMPS


*Structure of the TrkH potassium transporter from Cao Y, Jin X, Huang H, Derebe MG, Levin EJ, Kabaleeswaran V, Pan Y, Punta M, Love J, Weng J, Quick M, Ye S, Kloss B, Bruni R, Martinez-Hackert E, Hendrickson WA, Rost B, Javitch JA, Rajashankar KR, Jiang Y, Zhou M. "Crystal structure of a potassium ion transporter, TrkH." Nature 471:336-40, 2011. Pubmed ID:21317882 | Search SBKB Publications Portal